The term ICU acquired infection refers to an infection that was not present during admission to the ICU, and that may occur after discharge from ICU. It is estimated that 10-25% of persons are affected by nosocomial infection in India each year. The nurses can play a major role in preventing the hospital acquired infections thorough knowledge regarding modes of spreading and control measures. The research approach adopted for the study was evaluative approach and the research design was Quasi-experimental one group pretest /post-test design. The sample of the study was newly appointed staff nurses working in K.C.G Hospital, Bangalore.