Background: The objective of this laboratory-based study was to assess how different endodontic irrigants affect the micro-tensile bond strength (µTBS) of one-step self-etch adhesive systems to dentin surfaces. Material and methods: Forty-five intact human molars were utilized in this experiment, divided into three groups based on the type of irrigants used. A self-etch universal adhesive system was applied for composite restoration in all groups. Results: the groups treated with EDTA + NaOCl displayed notably reduced bond strength compared to the other two groups. Conclusion: Despite the constraints of our study, the control group demonstrated markedly lower bond strength compared to the comparative groups.