Objective(s): This study aims to assess the students' level of ambition and psychological and academic alienation among nursing students and identify the relationship between them. Methodology: A descriptive design used in the present study was established for the period from November 2022 to June 2022. A convenient “non- probability" sample, of (300) nursing student were selected from college of nursing university of Baghdad and department of nursing in Al- Hadi university college. The instrument of the study is adopted for the purpose of this study which consist of the socio- demographic data, psychological alienation scale and level of ambition scale, the data were analyzed through the application of descriptive and inferential statistical approaches which are applied by using IBM/SPSS package version 24.0.