Operculina turpethum linn has been traditionally claimed that the roots of Operculina turpethum linn are useful in the management of wound healing. The roots of Operculina turpethum linn are reported to have anti-inflammatory, antioxidant, and antimicrobial properties. In the present study, wound healing activity of ethanolic extracts of Operculina turpethum linn was evaluated in rats using excision wound model along with estimation of phytochemical parameters. In the excision wound model, the period of epithelisation, scar area, percent wound contraction or closure was evaluated respectively. The treatment of diabetic wound with oral containing ethanolic extracts of Operculina turpethum linn exhibited significant wound healing. The results were comparable to standard drug povidone iodine ointment, in terms of percentage of wound contraction, parameters. A significant antioxidant activity was observed as evidenced from decreased level of MDA and increased the level of GSH, catalase in extract treated animals as compared to control animals.