The primary source of medicine for underprivileged populations without access to contemporary medical care is medicinal plants. The phytochemical and biological screening of several plants obtained from Dhunkharka village in the Kavrepalanchowk district of Nepal is the subject of this study. Stephanie Flavanoids and glycosides were abundant in glandulifera, Cuscuta reflexa, Bergenia ciliata, Melia azadirachta, Drymaria diandra, Jasminum humile, Astelbe rivularis, Oxalis corniculata, and Viola serpens. Methanolic extract was employed for antibacterial and phytochemical screening purposes. Even though the Dhunkharka population uses a variety of plants, only an extract from Oxalis corniculata and Jasminum humile had a minor antibacterial action on the human pathogenic bacterial strain E. coli. Although the sensitivity of each bacterial strain varies, the methanolic extract of Astelia rivularis had the strongest antibacterial efficacy against Escherichia coli.