This study aimed to investigate the effectiveness of a brain-based learning program in improving mathematical skills among students with learning difficulties in mathematics. The program consisted of 16 training sessions. The research used a semi-experimental design. In order to achieve this aim, the researcher developed a math skills test. The population of the study consisted of all students with difficulties in learning mathematics in grades four-six who are enrolled in the learning resource rooms in the schools of the Directorate of Education in the Markka district in the second semester of the academic year 2022/2023. 50 students participated in the study, they were divided into two groups (experimental group = 26 ،control group = 24). The results revealed the effectiveness of a brain-based learning program in acquiring mathematical skills for students with learning difficulties.