This current study spots the light over the impact of human resources management on knowledge management at the Jordanian Universities, whereas the study sample consisted of (662) respondents by utilizing the quantitative approach, and a questionnaire was designed to perform the collecting data process. Additionally, the questionnaire included the two main scales of the study: Human resources management strategies, and Knowledge management, also the sample distribution was based on the following variables: (Gender, Experience, Academic position, and College). Indeed, the results showed that all the following areas: (Planning, Performance effectiveness & Quality, Training & development, Bounties & compensations, and Evaluation) for human resources management strategies came in a high degree, similarly, the results showed that all the following areas: (Obtaining & developing knowledge, Organize & save knowledge, Transfer & publish knowledge, and Applying knowledge) for knowledge management came in a high degree. Finally, the results indicated there are a statistically significant effect at the level (0.05) for human resources management on knowledge management in the Jordanian universities.