This research article aims to investigate the impact of tourism behavior on the sustainability of nature tourism in Cyprus. In particular, it identifies the factors that contribute to unsustainable tourism behavior in natural areas and assess their impact on the environment. Deploying a two-phase mixed methods approach, the study administered a survey of tourists visiting nature-based attractions in Cyprus in the first phase, and an in-depth interview with key stakeholders involved in nature tourism. The results of the study indicate that tourism behavior has a significant impact on the sustainability of nature tourism in Cyprus.