The purpose of this article is to show the relationship and interaction of the Sheibanid rulers with the local Maverannakhr Sufi brotherhoods - Naqshbandiyya, Kubraviyya and Jahriya (Yassaviyya). The main materials for this study were information from Persianlanguage historical sources and Muslim hagiographic works (manakib) of the 16th century. The second generation of Sheibanids, whose representatives came to power in the mid-30s of the 16th century, unlike their predecessors, sought to establish trusting relationships with the leaders of various Sufi brotherhoods of Maverannahr.