Due to the development of numerous complications such as vascular damage, neuronal damage, nephronal damage, and retinal damage, both IDDM and NIDDM are linked to increased mortality and morbidity. The aim of the present study is to assess the acute and sub-acute toxicity of ethanolic PP extract. In the acute toxicity, at a dose of 500 , 1000 mg/kg was administered to wistar rat which was then observed for physical symptoms and behavioral changes. In sub -acute toxicity repeated doses of the polyherbal powder were administered to wistar rat. The animals received three doses of polyherbal powder (500 mg/kg, 750 mg/kg 1000 mg/kg , 2000 mg/kg ) for the period of 28 days. On 29th day of experiment, blood sampling of animals was done for hematological and biochemical analysis. There was no morbidity and mortality with single dose administration in acute toxicity study in rat. In sub -acute toxicity there was change in the body weight of the treated rat when compared to control rat, there was morphological changes in liver, kidney, stomach of the treated rat was recorded at a dose of 2000 mg/kg. From the data obtained in this study, it can be concluded that though LD50 is greater than 1500mg/ kg b.w. but moderate toxicity signs appeared in liver, kidney, stomach at limit dose.