During the winter agricultural season 2021-2022, a field experiment was conducted in one of the fields located in the Al-Hindiyah District of the Holy Karbala Governorate. The purpose of the experiment was to study potential mutations that could make plants resistant to the action of certain herbicides belonging to the genus Lolium. The experiment was designed according to the randomized complete block design (R.C.B.D) with split plots arrangement and three replications, as the main plots included four different types of herbicides, namely Chevalier, Pallas, Tobik, and Axial, along with a comparison treatment (spraying with water only), while the sub plts included planting Lolium bush seeds. The holy province of Karbala, the province of Babylon, and the province of Wasit are the three provinces that make up this grouping in the Iraqi country of Iraq. The results revealed mutations in the ALS gene in the mRNA sequences of plants from Karbala, Wasit, and Babylon that are resistant to the pesticides Chevalier and Pallas, by substituting nitrogenous bases with other bases in codons 195 and 200 of AGC and AGT, which encode for the amino acid Ser to ACC, and GGT, which encodes for Thr and Gly. Iraqi weed mutations matched global mutations.