Nanoparticles are microscopic particles smaller than 100μm. Nanoparticles was synthesized usingvarious metals. The nanoparticles synthesized with silver nitrate were preferred for numerous applications.In the present study wheatgrass was used as herbal medium. Wheatgrass has pharmacological andmedicinal properties. The nanoparticles were synthesized by using incubation process. After addition of1mM AgNO3 in wheatgrass extract, the extract becomes brown indicated the synthesis of nanoparticles.The synthesized silver nanoparticles showed maximum absorption at 420nm indicated the formation ofnanoparticles. The synthesized nanoparticles were showed considerable effect on seed germination rateand nodule formation. As well as the synthesized nanoparticles was showed countable antimicrobialactivity against gram positive, gram negative and fungus.